烟粉虱对双丙环虫酯的田间抗性监测及抗性风险评估

Field monitoring and risk assessment of the resistance in tobacco whitefly Bemisia tabaci to afidopyropen

中文关键词:烟粉虱  双丙环虫酯  抗性监测  风险评估  交互抗性

keywords:Bemisia tabaci  afidopyropen  resistance monitoring  risk assessment  cross resistance

中文摘要:为明确烟粉虱Bemisia tabaci对双丙环虫酯的田间抗性现状及潜在抗性风险，通过浸叶法测定北京、天津、河北、辽宁和河南省（市）9个地区烟粉虱田间种群对该药剂的抗性水平，并对室内敏感种群进行连续15代抗性筛选，估算抗性现实遗传力h²，预测抗性发展速率，同时检测对阿维菌素、溴氰虫酰胺、噻虫嗪、氟啶虫胺腈、氟吡呋喃酮和联苯菊酯6种杀虫剂的交互抗性。结果显示：北京通州、北京昌平、北京平谷、天津西青、天津武清、张家口崇礼、张家口怀来和辽宁铁岭8个种群对双丙环虫酯的抗性倍数为1.1～2.7倍，处于敏感水平；河南杞县种群对其的抗性倍数为3.9倍，处于敏感性下降阶段。经过连续15代抗性筛选，烟粉虱SEL-R种群对双丙环虫酯的抗性倍数为5.4倍，h²为0.107；在50%、60%、70%、80%和90%的选择压下，抗性上升10倍所需的代数分别为22.09、18.15、15.12、12.60和10.00代；抗性上升100倍所需的代数分别为44.18、36.30、30.25、25.20和20.00代。双丙环虫酯抗性种群对阿维菌素、溴氰虫酰胺、噻虫嗪、氟啶虫胺腈、氟吡呋喃酮和联苯菊酯的抗性倍数为0.9～1.2倍，无交互抗性。综上，烟粉虱对双丙环虫酯存在一定抗性风险，田间应合理控制用量与施药频次，建议与不同作用机制杀虫剂混用或轮用。

Abstract:To clarify the current field resistance status and potential risk of resistance in tobacco whitefly Bemisia tabaci to afidopyropen, the leaf-dip method was used to determine the resistance levels of field populations collected from nine regions across Beijing, Tianjin, Hebei, Liaoning, and Henan provinces/municipalities. A susceptible laboratory strain was subjected to continuous resistance selection with afidopyropen for 15 generations to estimate the realized heritability(h²) and predict the rate of resistance development. Cross-resistance to six insecticides(abamectin, cyantraniliprole, thiamethoxam, sulfoxaflor, flupyradifurone, and bifenthrin) was also evaluated. The results indicated that the resistance ratios of eight populations—Tongzhou(Beijing), Changping(Beijing), Pinggu(Beijing), Xiqing(Tianjin), Wuqing(Tianjin), Chongli(Zhangjiakou), Huailai(Zhangjiakou), and Tieling(Liaoning)—ranged from 1.1 to 2.7-fold(<3-fold), suggesting susceptible levels. The population from Qixian(Henan) exhibited a resistance ratio of 3.9-fold, indicating a stage of reduced sensitivity. After 15 generations of resistance selection, the resistance ratio of B. tabaci SEL-R population to afidopyropen reached 5.4-fold, with an h² value of 0.107. Under selection pressures of 50%, 60%, 70%, 80%, and 90%, the numbers of generations required for a 10-fold increase in resistance were 22.09, 18.15, 15.12, 12.60, and 10.00 generations, respectively; for a 100-fold increase, the corresponding numbers were 44.18, 36.30, 30.25, 25.20, and 20.00 generations, respectively. The afidopyropen-selected strain showed cross-resistance ratios ranging from 0.9 to 1.2(<3-fold) to the six insecticides, indicating an absence of cross-resistance. In conclusion, B. tabaci poses a discernible risk of developing resistance to afidopyropen. Therefore, the application dosage and frequency in the field should be rationally regulated, and it is advisable to use afidopyropen in rotation or combination with insecticides featuring distinct modes of action.