我国黄淮海地区大豆症青相关病毒的鉴定及其抗（耐）性材料筛选

Identification of soybean stay-greenassociated virus in the Huang-Huai-Hai region of China and screening of disease-resistant soybean materials

中文关键词:大豆  症青  大豆症青相关病毒  普通棕色叶蝉  抗性鉴定

keywords:soybean  stay-green syndrome  soybean stay-green associated virus  common brown leafhopper  resistance identification

中文摘要:为探究黄淮海地区大豆症青连年暴发的原因并筛选优异的抗（耐）性大豆新品种（系），在黄淮海地区不同地区采集疑似病样209份，利用特异性引物对病样的叶片和豆荚果皮进行大豆症青相关病毒（soybean stay-green associated virus，SoSGV）的检测，随机选取21个阳性样品进行全基因组测序及系统发育分析，构建SoSGV侵染性克隆进行侵染性试验，并以普通棕色叶蝉Orosius orientalis为SoSGV传毒介体对274份我国新育成大豆品种（系）进行抗性评价。结果显示：采集自黄淮海各省份表现症青的大豆病样中SoSGV的检出率均超过70%，甚至在个别省份检出率达100%，初步推断SoSGV是我国黄淮海地区大豆症青的主要病原，且该病害有向西北大豆产区扩散的趋势；21条阳性样品的SoSGV全基因组与已知的6条SoSGV全基因组间的核苷酸序列同源性均超过92%，系统发育分析显示该病毒共有3个进化群体，以群体I为主要流行种群；与其他双生病毒的系统发育分析显示SoSGV属于一种新型重组双生病毒；构建的SoSGV侵染性克隆可成功侵染本氏烟Nicotiana benthamiana；在274份大豆新品种（系）中有271份对SoSGV表现为易感，且出现典型症青症状，仅有3份为耐病新品种（系）。

Abstract:To investigate the causes of recurrent outbreaks of soybean stay-green syndrome in the Huang-Huai-Hai region and to screen soybean cultivars (lines) with resistance (or tolerance) to this disease, a total of 209 suspected samples were collected from different provinces within the region. Specific primers were used to detect soybean stay-green associated virus (SoSGV) in the leaves and pod pericarp tissues of the collected samples. Whole-genome sequencing and phylogenetic analysis were conducted on 21 randomly selected SoSGV-positive samples, and infectious clones of SoSGV were constructed for infectivity assays. Additionally, 274 newly developed soybean cultivars (lines) in China were evaluated for resistance to SoSGV using the common brown leafhopper Orosius orientalisi as the transmission vector. The results revealed that SoSGV detection rates exceeded 70% in samples collected across provinces in the Huang-Huai-Hai region, with some provinces reaching 100%. This result suggested that SoSGV was the primary pathogen causing soybean stay-green syndrome in China, with a trend of spread toward northwestern soybean-producing areas. The genome nucleotide sequences of 27 SoSGV isolates shared over 92% identity. Phylogenetic analysis identified three evolutionary groups, with group I as the predominant epidemic population. Comparative phylogenetic analysis with other geminiviruses revealed that SoSGV was a novel recombinant geminivirus. Infectious clones of SoSGV successfully infected Nicotiana benthamianai. Among the 274 soybean cultivars (lines) evaluated, 271 were highly susceptible to SoSGV and exhibited typical stay-green symptoms, while only three cultivars (lines) showed resistance to this disease.