干扰VmCarEs-6基因影响苜蓿斑蚜对高效氯氟氰菊酯的敏感性

Interference with the VmCarEs-6 gene affects the sensitivity of spotted alfalfa aphid Therioaphis trifolii to lambda-cyhalothrin

中文关键词:苜蓿斑蚜  高效氯氟氰菊酯  RNA干扰  毒力测定  纳米材料

keywords:spotted alfalfa aphid  lambda-cyhalothrin  RNAi  toxicity testing  nanometer material

中文摘要:为阐明VmCarEs-6基因在苜蓿斑蚜Therioaphis trifolii对高效氯氟氰菊酯抗性中的作用，于室内测定高效氯氰菊酯对苜蓿斑蚜的毒力和高效氯氰菊酯不同致死浓度胁迫后苜蓿斑蚜体内VmCarEs-6基因的相对表达量，以星状阳离子聚合物（star polycation，SPc）纳米材料作为载体将dsVmCarEs-6导入到苜蓿斑蚜体内，测定干扰效率和苜蓿斑蚜的存活率，并测定干扰VmCarEs-6基因后高效氯氰菊酯对苜蓿斑蚜的室内毒力。结果表明：随着高效氯氟氰菊酯浓度和处理时间的增加，其对苜蓿斑蚜的致死效果越显著；高效氯氟氰菊酯对苜蓿斑蚜的致死浓度LC₁₀、LC₅₀和LC₇₀分别为2.550、5.617和7.761 mg/L；LC₁₀、LC₅₀和LC₇₀高效氯氟氰菊酯处理48 h后，苜蓿斑蚜体内VmCarEs-6基因的相对表达量均显著高于对照；随着处理时间增加，dsVmCarEs-6的干扰效果越显著，dsVmCarEs-6处理48 h后，苜蓿斑蚜体内VmCarEs-6基因的相对表达量较对照组极显著降低，干扰效率为73.30%；dsVmCarEs-6处理6、12、24、36和48后，苜蓿斑蚜的存活率分别为92.22%、88.89%、86.67%、83.33%和78.89%，均与对照无显著差异；dsVmCarEs-6处理48 h后，不同浓度高效氯氟氰菊酯处理的苜蓿斑蚜死亡率均较对照显著增加，表明苜蓿斑蚜VmCarEs-6基因影响苜蓿斑蚜对高效氯氟氰菊酯的敏感性。

Abstract:To elucidate the role of VmCarEs-6 gene in the resistance of the spotted alfalfa aphid Therioaphis trifolii to high-efficiency cypermethrin, the toxicity of high-efficiency cypermethrin to alfalfa aphids and the relative expression of VmCarEs-6 gene in alfalfa aphids under different lethal concentrations of high-efficiency cypermethrin stress were measured indoors. Star polycation (SPc) nanomaterials were used as carriers to introduce dsVmCarEs-6 into spotted alfalfa aphids, and the interference efficiency and survival rate of alfalfa spotted aphids were determined. The indoor toxicity of lambdacyhalothrin to spotted alfalfa aphids after interference with VmCarEs-6 gene was also measured. The results showed that as the concentration and treatment time of lambda-cyhalothrin increased, its lethal effect on spotted alfalfa aphids became more significant. The lethal concentrations (LC₁₀, LC₅₀, and LC₇₀) of lambda-cyhalothrin against alfalfa aphids were 2.550, 5.617, and 7.761 mg/L, respectively. After 48 h of treatment with lethal concentrations of LC₁₀, LC₅₀, and LC₇₀ of lambda-cyhalothrin, the relative expression levels of VmCarEs-6 gene in spotted alfalfa aphids were significantly higher than those in the control group. As the processing time increased, the interference effect of dsVmCarEs-6 became more significant. After 48 h of treatment with dsVmCarEs-6, the relative expression level of the VmCarEs-6 gene in spotted alfalfa aphids was significantly reduced compared to the control group, with an interference efficiency of 73.30%; After treatment with dsVmCarEs-6 at 6, 12, 24, 36, and 48, the survival rates of spotted alfalfa aphids were 92.22%, 88.89%, 86.67%, 83.33%, and 78.89%, respectively, with no significant difference compared to the control. However, After 48 hours of treatment with dsVmCarEs-6, the mortality rate in different concentrations of lambda-cyhalothrin treatment groups significantly increased compared with the control group (CK). These results indicate that the VmCarEs-6 gene affects the sensitivity of alfalfa aphids to lambda-cyhalothrin.