冷蒿粗提物协同金龟子绿僵菌对亚洲小车蝗存活及生长发育的影响

Synergistic efficacy of prairie sagebrush Artemisia frigida crude extract and entomopathogenic fungus Metarhizium anisopliae on the development of resistant grasshopper Oedaleus decorus asiaticus

中文关键词:蒿属植物  杀虫真菌  协同防治  生物农药  蝗虫  复配

keywords:Artemisia  insecticidal fungi  synergetic control  biopesticide  grasshopper  mixed formula

中文摘要:为探究冷蒿Artemisia frigida粗提物与金龟子绿僵菌Metarhizium anisopliae对亚洲小车蝗Oedaleus decorus asiaticus的协同防治效果，用不同浓度的冷蒿粗提物和金龟子绿僵菌复配饲喂亚洲小车蝗4龄蝗蝻后进行毒力测定，并采用室内生测法测定两者对其生长发育的影响。结果显示：10%冷蒿粗提物与10⁸个孢子/g的金龟子绿僵菌复配处理后亚洲小车蝗的死亡率最高，为82.5%，显著高于其余复配处理、单独使用冷蒿粗提物和单独使用金龟子绿僵菌处理后的死亡率；10%和20%的冷蒿粗提物分别与10⁸个孢子/g的金龟子绿僵菌复配处理后，亚洲小车蝗4龄蝗蝻的体重增加量、体长增加量、生长速率以及总体表现力均显著优于单独使用冷蒿粗提物和单独使用金龟子绿僵菌的效果，其中10%冷蒿粗提物与10⁸个孢子/g金龟子绿僵菌复配处理的效果最佳。表明冷蒿粗提物与金龟子绿僵菌复配对抑制亚洲小车蝗存活和生长发育具有协同和拮抗作用，有开发为新型生物农药的潜力。

Abstract:In order to explore the synergistic control effect of crude extracts of prairie sagebrush Artemisia frigida and entomopathogenic fungus Metarhizium anisopliae on grasshopper Oedaleus decorus asiaticus, we used different doses of M. anisopliae and crude extracts of A. frigida singly and in combination, to determine their toxicities and the effect of growth and development to fourth instar O. decorus asiaticus. The results showed that the combination of 10% crude extract of A. frigida with 10⁸ spores/g M. anisopliae concentrations had significant effects on the mortality of O. decorus asiaticus with 82.50% than those of the crude extract of A. frigida and M. anisopliae alone and other compounding treatments. The 10% crude extract of A. frigida associated with 10⁸ spores/g M. anisopliae and the combination of 20% crude extract of A. frigida with 10⁸ spores/g M. anisopliae concentrations had significant effects on the body weight and length gain, growth rate and overall performance of female O. decorus asiaticus than those of the crude extract of A. frigida and M. anisopliae alone. Among them, the 10% A. frigida crude extract mixed with 10⁸ spores/g M. anisopliae had the maximal control efficacy. The results indicated that the combinatorial treatment had synergistic and antagonistic effects on host mortality, growth, and development in O. decorus asiaticus, having a potential to be developed as a new type of biopesticide for sustained control of grasshoppers.